Introduction
DNA laboratory testing is an integral part of genetic analysis. A thorough, reliable, and correct study of the genome allows not only the identification of its qualitative composition but also the identification of potential abnormalities. It should be pointed out that DNA is genomic material inherent in many living organisms, except those whose genome is based on RNA. In addition, DNA within living cells is not universal, especially in the case of bacteria; most of them have both chromosomal DNA chains and ring plasmids. In short, the present work explored the possibilities of genetic analysis to determine the chromosomal DNA of the bacterium E. coli.
Selected Material
E. coli was chosen as the bacterial organism whose chromosomal DNA was isolated by Lab. 8. The bacterial DNA was then removed from the cell and prepared (purified) for analysis. In this part of the work, the prepared DNA was used for several analysis phases to determine the identity and purity of the resulting material.
The essence of the work
Two parts of the study were used during the current analysis: the preparation of DNA by enzymatic processing in a centrifuge and the use of gel electrophoresis. With this arrangement, it is possible to obtain a gel image of the DNA, according to which it is possible to infer the purity and nature of the isolated material. The use of a negative control simplifies the analysis by comparing the two bands. In particular, a non-DNase-treated sample will give stripes on the gel image because this enzyme breaks DNA by cleaving the phosphodiester bond in the molecules.
Conclusion
Thus, the overall goal of the present work was, to utilize gel electrophoresis as an instrumental analysis, to determine the identity and purity of previously isolated bacterial DNA.