Hypothesis and objectives
The experiment aimed to test the reaction of bacteria towards some antibiotics and determine effectiveness of those antibiotics in treating some diseases. The effectiveness of the antibiotic was determined by carrying an experiment on three bacteria under the same temperature.
The hypothesis of the experiment was:
- Ho: The entire four antibiotics react with bacteria.
- HA : bacteria resist some of the antibiotics.
The experiment method will be used throughout the study. Data was collected through the experiment. This helped me enable to establish the theoretical framework of my experiment.
Analysis of the data
Pseudomonas aeruginosa is hard to treat because they produce extended-spectrum beta-lactamase and inducible clindamycin which offers resistance to antibiotics. In the experiment out of the four antibiotics used, Pseudomonas aeruginosa was sensitive to the one, Gentamicin whose zone of inhibitions was 15mm while their inhibition zoning was 0mm. this means the antibiotics used in the tests will work on Pseudomonas aeruginosa. Therefore there is only one antibiotic that will work on this type of bacteria as shown in the experiment is Gentamicin. Chloramphenicol antibiotic is used to destroy the bacteria. This drug works well where it is intended is inhibit protein synthesis by binding 50s ribosome.
Four antibiotics have been tested against staphylococcus aureus where all of them have proved sensitive but with different levels of inhibition zoning. Ampicillin is the most effective with an inhibition zoning of 35mm, erythromycin has an inhibition zoning of 32mm, Oxacillin inhibition zoning of 23mm and Bacitracin inhibition zoning of 21mm. all antibiotics tested here were effective but some acted first than others as shown from their inhibition zoning. These drugs are effective in these bacteria as they do not offer any resistance to them.
In the case of Klebsiella pneumonia bacteria two antibiotics worked well two of them did not work. There was one on Ampicillin and Bacitracin where their inhibition zoning was 0mm. while cephalothin and gentamicin had inhibition zoning of 20mm and 34mm respectively. From this experiment some of the drugs that can be used to treat MRSA are cephalothin of Cephalosporins drug class and generation of Keflex with a zoning of 20mm. the other drug is Gentamicin with inhibition zoning of 34mm from drug class of Aminoglycosides. The drugs that were experimented with in this class but was not effective were Bacitracin whose popular brand name is Neosporin.
Conclusion
From the experiment it can be concluded that in order to treat Pseudomonas aeruginosa, gentamicin should be used which is drugs class of Aminoglycosides and inhibits bacterial protein synthesis. This drug is effective unless the bacterium develops resistance to it as time goes by. However the treatment of MRSA has two drugs that are effective as shown by the experiment that is cephalothin and gentamicin. The treatment of staphylococcus aureus is easier as most drugs tested proved sensitive and had a large inhibition zoning. Some of these drugs that proved effective included, erythromycin, Oxacillin, Ampicillin and Bacitracin.
To treat any illness the right kind of drugs should be administered to help most people recover and lead healthy, productive lives; wrong medication will lead to resistance to drugs. Drug naming, packaging and labeling and terms used in them should also be clear. This will reduce cases of wrong medications and help avoid future drug resistance.