The research mainly focuses on intercultural communication experiences between non-native English-speaking women and native English speakers. Thus, the purpose of the study is to examine the work interaction experience of these groups in order to understand the underlying barriers of communication. The major finding is the fact that both groups face similar communication barriers when interacting with each other, but one’s accent can also be a hindrance. The argument is that intercultural communication can lead to a number of communication challenges, such as accents, misunderstanding, and differences in directness. The conclusion revolves around suggesting organizations to create a culture or environment that empowers all cultural differences. The greatest strength of the research is its narrow focus on professional women, which eliminates factors of sexism, gender gap, and racism for the project. The main weakness is the smallness of the sample size, and the study’s strength can generate a bias when applied to the working men. The resource will be helpful in widening the overall understanding of the role of intercultural communication barriers in assessing organizational culture. Therefore, the relevance will be found in identifying various communication challenges in an organization.